Purpose: This study aims to explore how Human Resource Management practice can promote environmental sustainability in Higher Education Institutions (HEIs) in Karachi, Pakistan and also investigates the role of organizational culture in supporting eco-friendly behaviors among employees.

Design: A mixed-methods approach was adopted with HR professionals and faculty members from various HEIs in Karachi.

Findings: The study found a positive relationship between HRM practices and the implementation of sustainability initiatives within HEIs and significantly influence eco-friendly behaviors environmental outcomes and a strong organizational culture mediates the effectiveness of HRM practices in achieving sustainability goals.

Practical Implications: The findings offer practical recommendations for HEIs in resource-constrained environments, suggesting that even low-cost or incremental HRM interventions can positively impact sustainability.

Originality: Contributes to the literature by examining the intersection of HRM and sustainability of HEIs in a developing region. It provides insights into the unique challenges faced by HEIs in Karachi.